MOLECULAR TYPING OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS BY PCR-RFLP OF SPA GENE: A REFERENCE LABORATORY PERSPECTIVE
نویسندگان
چکیده
منابع مشابه
Molecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective.
PURPOSE To characterize methicillin-resistant Staphylococcus aureus (MRSA) strains by molecular typing based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of spa gene and to assess the utility of spa genotyping over bacteriophage typing in the discrimination of the strains. MATERIALS AND METHODS Studies were undertaken on 125 MRSA strains representing the mo...
متن کاملMolecular typing of methicillin-resistant Staphylococcus aureus by PCR-RFLP and its usefulness in an epidemiological study of an outbreak.
A new convenient molecular typing method, simultaneous polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis, for three different genes of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated using 35 isolates of MRSA and comparing results with those previously reported for sequencing-based spa typing. Twenty-nine isolates of the most frequent prot...
متن کاملDetection anD typing methoDs of methicillin-resistant StaphylococcuS aureuS strains
Staphylococcus aureus is one of the most important pathogens in hospital infections and its importance in the community is also prominent, especially since the 1990’s. Today methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of hospital infection, causing 40-70% of staphylococcal infections in intensive care units. Data on the prevalence of MRSA isolates differ ...
متن کاملmolecular typing of methicillin-resistant and methicillin-susceptible staphylococcus aureus isolates from shiraz teaching hospitals by pcr-rflp of coagulase gene.
background and objectives: to investigate coagulase gene polymorphisms of mrsa and mssa isolates from shiraz teaching hospitals from 2011 to 2012. materials and methods: a total of 302 isolates of staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. the isolates were subj...
متن کاملMolecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene
BACKGROUND AND OBJECTIVES To investigate coagulase gene polymorphism of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. MATERIALS AND METHODS A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subje...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2009
ISSN: 0255-0857
DOI: 10.4103/0255-0857.45363